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Can the gut microbiota be a guide in the diagnosis and treatment of childhood epilepsy?

Objectives: To contribute the role of the gut-brain axis in the pathogenesis of epilepsy and to find biomarkers that will help determine new strategies in this regard. Methods: Twenty children with epilepsy of ‘unknown etiology’ and seven healthy controls in the same age group were included in the study. The groups were compared with questionnaire. Stool samples were kept in a tube containing DNA/RNA Shield™ with a sterile swab. Then, sequencing was done with MiSeq™System(Illumina). 16SrRNA sequencing of samples using next-generation sequencing;V4 variable region PCR amplification was concluded by 2x250bp paired-end sequencing of amplicons and at least 50,000 reads(>Q30) per sample. The DNA sequences taken from here were classified at the genus level using the Kraken program. Then bioinformatics and statistical analysis were conducted. Results: There was no difference between the groups sociodemographic characteristics, medical and family history, dietary habits. Gut microbiota(GM) composition analysis, there were no difference between the groups in terms of alpha and beta diversity(Figure 1,2). Relative abundance values of GMs of individuals differed between groups at the level of genus, order, class, family, filum. Flavihumibacter, Niabella, Anoxybacillus, Brevundimonas, Devosia, Delftia were seen only in the control group; Megamonas and Coriobacteria were only seen in the epilepsy group(Figure 3,4,5). As a result of the LEfSe method, it was determined that 33 taxa were important in separating the groups(Figure6). The heatmap graph created are added in Figure7. Conclusion:We think, bacterial varieties(especially megamonas and coribacteria) that differ between both groups can be used as biomarkers in treatment and diagnosis.
Keywords: gut microbiota, chilhood epilepsy, biomarker, diagnosis, treatment

Sevim Türay
Duzce University Faculty of Medicine
Turkey

Şengül Cangür
Duzce University Faculty of Medicine
Turkey

Gözde Kahraman
Duzce University Faculty of Medicine
Turkey

Eda Kayabaşı
Duzce University Faculty of Medicine
Turkey

Ömer Faruk Çetiner
Duzce University Faculty of Medicine
Turkey

Burak Aydın
Health Sciences University
Turkey

Cihadiye Elif Öztürk
Istanbul Arel University, Faculty of Medicine
Turkey

 

 


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